Synthesis and characterization of the ideal methacrylation degree for Methacryloyl Gelatin (GelMA)
DOI:
https://doi.org/10.18779/ingenio.v6i2.676Keywords:
methacrylic anhydride, spectroscopy, gelatinee methacryoyl, morphology, timeAbstract
The purpose of this study was to synthesize methacryloyl gelatin by modifying type A gelatin from porcine skin with methacrylic anhydride based on the reaction technique using phosphate saline solution, there were two variable parameters that are methacrylic anhydride and the time of reaction. A total of six samples were obtained that were studied through characterization tests to confirm GelMA formation and morphological analysis. In the infrared spectroscopy with Fourier transform, the chemical groups of each sample were shown, highlighting the peaks of the functional amino groups NH, amides I, II and III. In scanning electron microscopy, the morphology of methacryloyl gelatin and its porous nature were analyzed, obtaining a sample with an average pore size of approximately 150 microns, which will benefit oxygen diffusion and support cell growth; the contact angle of the samples is in a range of 53º and 65º, therefore they have a high degree of wettability.
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