Analysis of environmental DNA in the determination of fertility of agricultural soil in the province of Loja
DOI:
https://doi.org/10.18779/ingenio.v6i1.558Keywords:
Bacterial Biomass, Bacterial Counts, Soil Fertility IndexAbstract
Soil fertility can be determined through different processes, one of these is through the analysis of environmental DNA (eDNA) which allows identifying the genetic diversity found in said material, essential in the formation and maintenance of soil productivity. soil, this study is the first of its kind in the province of Loja, it was carried out by selecting 9 areas, in which 26 soil samples were collected, eDNA was measured and the number of microorganisms was estimated from of the amount of eDNA obtained. 35% of total soils (9) showed bacterial counts greater than 6.0 x 108 cells/g-soil, 15% (4) between 2.0 x 108 cells/g-soil (minimum bacterial counts required for the mineralization of organic compounds) and 6.0 x 108 cells/g-soil. However, the remaining 50% (13) had less than 2.0 x 108 cells/g-soil in these soils mineralization with organic nitrogen is extremely low, found in the Saraguro, Paltas and Catamayo cantons. This study is important because it identifies the bacterial counts of different soils and the need to incorporate microorganisms to improve yields, thanks to standardization, the technique can be applied to all the soils of the province and the country.
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M. Kubo, M. Mukai, y D. Adhikari Construction of Soil Fertile Index (SOFIX) Based on Microorganisms and Application for Agriculture. Journal of Environmental Biotechnology, 15(2): 85-90 2016
A.C. Kennedy, Bacterial diversity in agroecosystems. Agriculture, Ecosystems & Environment. Elsevier volume 74: 65-76. 1999
A Sharpley, Agricultural phosphorus, water quality, and poultry production: are they compatible?. Poultry Science. Volume 78, Issue 5,1999, Pages 660-673
D. Rigby, D. Cáceres, Organic farming and the sustainability of agricultural systems. Agricultural Systems. Volume 68, Issue 1, 2001. Pages 21-40
P. Maeder, A. Fliessbach, D. Dubois, L. Gunst, P. Fried and U. Niggli. Soil fertility and biodiversity in organic farming. Science 296: 1694-1697. 2002
M.A. Tsiafouli, E. Thébault, S.P. Sgardelis, P.C de Ruiter, W.H. van der Putten, K .Birkhofer, L. Hemerik, F.T. de Vries, R.D. Bardgett, M.V. Brady, L. Bjornlund, H.B. Jørgensen, S. Christensen , T.D. Hertefeldt, S. Hotes, W.H. Gera Hol, J. Frouz, M. Liiri, SR Mortimer, H. Setälä, J. Tzanopoulos, K. Uteseny, V. Pižl, J. Stary, V.Wolters, K. Hedlund. Intensive agriculture reduces soil biodiversity across Europe. Glob Chang Biol. Feb;21(2):973-85 2015
R.J. Stirzaker, J.B. Passioura & Y. Wilms. Soil structure and plant growth: Impact of bulk density and biopores. Plant Soil 185, 151–162. 1996
E.K. Bünemann, L.M. Condron. Phosphorus and Sulphur Cycling in Terrestrial Ecosystems. In: Marschner, P., Rengel, Z. (eds) Nutrient Cycling in Terrestrial Ecosystems. Soil Biology, vol 10. Springer, Berlin, Heidelberg. vol. 10. Springer. 2007
H.W. Scherer. Sulfur in soils. Journal of Plant Nutrition and Soil Sci, 172(3): 326-335. 2009.
R. Martens. Current methods for measuring microbial biomass C in soil: Potentials and limitations. Biol Fertil Soils 19, 87–99. 1995
M.E. Arias, J.A. González-Pérez, F.J. González-Vila, y A.S. Ball. Soil health—a new challenge for microbiologists and chemists. Int Microbiol, 8(1): 13-21. 2005.
S. Tsuji, M. Ushio, S. Sakurai, T. Minamoto, y H. Yamanaka Water temperature- dependent degradation of environmental DNA and its relation to bacterial abundance. PLoS One, 12(4): e0176608. 2017
H. Aoshima, A. Kimura, A. Shibutani, C. Okada, Y. Matsumiya, M. Kubo. Evaluation of soil bacterial biomass using environmental DNA extracted by slow-stirring method. Appl Microbiol Biotechnol. Aug;71(6):875-80. 2006
D. N. Miller, J.E. Bryant, E.L. Madsen, y W.C. Ghiorse. Evaluation and optimization of DNA extraction and purification procedures for soil and sediment samples. Appl Environ Microbiol, 65(11): 4715-4724. 1999
J. Gans, M. Wolinsky, y J. Dunbar. Computational improvements reveal great bacterial diversity and high metal toxicity in soil. Science, 309(5739): 1387-1390. 2005
M. Kimura. Microbial World Acting in Soil (9) -Microorganisms in Grassland-. J. the Japanese Irrigation, Drainage and Reclamation Engineering, 59(12): 1413-1421. 1991.
H. Doi, R. Inui, Y. Akamatsu, K. Kanno, H. Yamanaka, T. Takahara, y T. Minamoto. Environmental DNA analysis for estimating the abundance and biomass of stream fish. Freshwater Biol, 62(1): 30-39. 2017.
J. Zhou, M.A. Bruns, y J.M. Tiedje. DNA Recovery from Soils of Diverse Composition. Appl Environ Microbiol, 62(2): 316-322. 1996
Y. Fukuhara, S. Horii, T. Matsuno, Y. Matsumiya, M. Mukai, y M. Kubo. Distribution of hydrocarbon-degrading bacteria in the soil environment and their contribution to bioremediation. Appl Biochem Biotechnol, 170(2): 329-339. 2013
S. Horii, T. Matsuno, J. Tagomori, M. Mukai, D. Adhikari, y M. Kubo. Isolation and identification of phytate-degrading bacteria and their contribution to phytate mineralization in soil. J Gen Appl Microbiol, 59(5): 353-360. 2013.
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