Isolation and Molecular Identification of Salmonella Spp., from Water Samples for Human Consumption

Authors

  • Favian Bayas-Morejón Centro de Investigación y Desarrollo Biotecnológico, Departamento de Investigación y Vinculación, Universidad Estatal de Bolívar, Facultad de Ciencias Agropecuarias, Recursos Naturales y del Ambiente, Guaranda 020150, Ecuador
  • Sonia Salazar-Ramos Centro de Investigación y Desarrollo Biotecnológico, Departamento de Investigación y Vinculación, Universidad Estatal de Bolívar, Facultad de Ciencias Agropecuarias, Recursos Naturales y del Ambiente, Guaranda 020150, Ecuador
  • Katherin Beltrán Centro de Investigación y Desarrollo Biotecnológico, Departamento de Investigación y Vinculación, Universidad Estatal de Bolívar, Facultad de Ciencias Agropecuarias, Recursos Naturales y del Ambiente, Guaranda 020150, Ecuador
  • Luis Verdezoto Centro de Investigación y Desarrollo Biotecnológico, Departamento de Investigación y Vinculación, Universidad Estatal de Bolívar, Facultad de Ciencias Agropecuarias, Recursos Naturales y del Ambiente, Guaranda 020150, Ecuador

DOI:

https://doi.org/10.18779/ingenio.v5i1.477

Keywords:

Salmonella spp., characterization, drinking water

Abstract

The objective of the study was to evaluate the occurrence of Salmonella spp. in 100 water samples, collected from five areas of the city of San José de Chimbo, prov. Bolivar. All the water samples were filtered using a suction ramp and membrane filters and then cultured using specific methods for the genus of interest and the isolates obtained were confirmed by microscopy and PCR (Polymerase Chain Reaction). After analyzes the pathogens were detected in 10% of the analyzed water samples. The highest number of contaminating samples was found in the sources of direct consumption, unlike the samples obtained directly from the slope that did not appear to be contaminated.

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Published

2022-01-13

How to Cite

Bayas-Morejón, F., Salazar-Ramos, S., Beltrán, K., & Verdezoto, L. (2022). Isolation and Molecular Identification of Salmonella Spp., from Water Samples for Human Consumption. InGenio Journal, 5(1), 65–70. https://doi.org/10.18779/ingenio.v5i1.477

Issue

Vol. 5 No. 1 (2022)

Section

Articles

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Copyright (c) 2021 Favian Bayas-Morejón, Sonia Salazar-Ramos, Katherin Beltrán, Luis Verdezoto

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