Literature Review for Counting Colony-Forming Units in Microorganisms Using Artificial Vision
DOI:
https://doi.org/10.18779/ingenio.v9i1.1117Keywords:
applied technology, artificial intelligence, image processing, bacterial cultures, automationAbstract
This study conducted a Systematic Literature Review with the aim of identifying the most efficient procedures for processing images used in microbiological analyses. This search includes publications from 2019 to 2025, using the main indexed scientific databases such as Scielo, ACM, Redalyc, Consensus, IEEE Xplore, and Springer, as well as the Google Scholar search engine. During the search, 41 articles were analyzed, identifying various approaches to traditional image processing, machine learning, and hybrid techniques. The results allowed us to identify methods and tools, providing a solid basis for their incorporation into a computer system. The main contribution is to establish the foundations for the development of an automated technological solution that facilitates the implementation of automatic counting to streamline the work of scientific and clinical laboratories by reducing time and human error.
Downloads
References
B. S. Cruz Florez, B. R. Gómez Torres, y L. C. Sanchez Leal, “Aplicaciones de la Inteligencia Artificial en Microbiología Agroambiental”, ReCIBE, Revista electrónica de Computación, Informática, Biomédica y Electrónica, vol. 13, no. 2, pp. C2-25, Sep. 2024. [En línea]. Disponible en: https://doi.org/10.32870/recibe.v13i2.362
P. J. Flores Medina, P. Garibay Murillo, y G. R. Peñaloza Mendoza, “Automation of inoculation in culture media for the microbiology laboratory”, Revista de Ciencias Tecnológicas, vol. 6, no. 4, Nov. 2023. [En línea]. Disponible en: https://doi.org/10.37636/recit.v6n4e285
M. Muñoz-Algarra, R. Martínez-Ruiz, y B. Orden-Martínez, “Evaluación del sistema automatizado UF-1000i en el diagnóstico de infección urinaria”, Enfermedades Infecciosas y Microbiología Clínica, vol. 31, no. 1, pp. 29–31, Jan. 2013. [En línea]. Disponible en: https://doi.org/10.1016/j.eimc.2012.05.017
R. Medeot, J. Mena, P. Rodríguez, P. Herrera Najum, L. López, y M. S. Muñoz, “Evaluaciones pre y post reprocesamiento manual y automatizado de endoscopios: aislamiento microbiológico”, Acta Gastroenterológica Latinoamericana, vol. 54, no. 3, pp. 254–261, Sep. 2024. [En línea]. Disponible en: https://doi.org/10.52787/agl.v54i3.423
E. P. Sánchez, D. Núñez, R. O. Cruz, M. A. Torres, y E. V. Herrera, “Simulación y Conteo de Unidades Formadoras de Colonias – Simulation and Counting of Colony-Forming Units”, ReCIBE, Revista Electrónica de Computación, Informática, Biomédica y Electrónica, vol. 6, núm. 1, pp. 97-111, Dec. 2017. [En línea]. Disponible en: https://doi.org/10.32870/recibe.v6i1.70
Cientisol, “Contadores de colonias: tipos y aplicaciones”, 2024. [En línea]. Disponible en: https://cientisol.com/contadores-de-colonias/
H. Munguía-Orozco, “Construcción de un modelo basado en redes neuronales profundas de código abierto para el conteo, localización y clasificación de colonias en placas de Petri”, ITESO, 2023. [En línea]. Disponible en: https://rei.iteso.mx/server/api/core/bitstreams/98df12f5-3c0e-4dcd-8c3d-e2ee2a0d77f9/content
M. T. Madigan, K. S. Bender, D. H. Buckley, W. M. Sattley, y D. A. Stahl, Brock Biology of Microorganisms, 15th ed. Boston, MA, USA: Pearson, 2021.
D. J. Hardy, “Practical Aspects and Considerations When Planning a New Clinical Microbiology Laboratory”, Clinics in Laboratory Medicine, vol. 40, no. 4, pp. 421-431, Dec. 2020. [En línea]. Disponible en: https://doi.org/10.1016/j.cll.2020.08.015
B. Kitchenham y S. Charters, Guidelines for performing Systematic Literature Reviews in Software Engineering, 2007.
A. Rehman, Z. Saleem, J. Amjad, S. R. Shah, y K. Siddique, “A Comparison of Bacterial Colonies Count from Petri Dishes Utilizing Hough Transform and Traditional Manual Counting”, arXiv, May. 2025. [En línea]. Disponible en: https://doi.org/10.48550/arXiv.2505.20365
S. Albaradei, F. Napolitano, M. Uludag, M. Thafar, S. Napolitano, M. Essack, V. B. Bajic, y X. Gao, “Automated counting of colony forming units using deep transfer learning from a model for congested scenes analysis”, IEEE Access, vol.8, pp. 164340-164346, Sep. 2020. [En línea]. Disponible en: https://doi.org/10.1109/ACCESS.2020.3021656
L. F. Coca, Z. P. Franco, y A. Pateti, “Implementación de filtros morfológicos utilizados en el procesamiento de imágenes digitales en un dispositivo lógico programable”, Univ. Cienc. Tecnol., vol. 12, no. 48, pp. 171–182, 2008. [En línea]. Disponible en: https://ve.scielo.org/scielo.php?pid=S1316-48212008000300008&script=sci_arttext
N. Triana, A. E. Jaramillo, R. M. Gutiérrez, y C. A. Rodríguez, “Técnicas de umbralización para el procesamiento digital de imágenes de GEM-Foils”, Scientia et Technica, vol. 21, no. 4, pp. 352–359, 2016. [En línea]. Disponible en: https://revistas.utp.edu.co/index.php/revistaciencia/article/view/13271
J. M. Llamas, “Reconocimiento de imágenes mediante redes neuronales convolucionales”, Tesis de grado, Universidad Politécnica de Madrid, Madrid, España, 2018. [En línea]. Disponible en: https://oa.upm.es/53050/
O. A. Soto Orozco, A. D. Corral Sáenz, C. E. Rojo González, y J. A. Ramírez Quintana, “Análisis del desempeño de redes neuronales profundas para segmentación semántica en hardware limitado”, ReCIBE. Rev. Electrón. Comput., Inform., Bioméd. Electrón., vol. 8, no. 2, 2020. [En línea]. Disponible en: https://doi.org/10.32870/recibe.v8i2.142
P. M. Rodrígues, J. Luís, y F. K. Tavaria, “Image Analysis Semi-Automatic System for Colony-Forming-Unit Counting”, Bioengineering, vol. 9, no. 7, p.271,2022. [En línea]. Disponible en: https://doi.org/10.3390/bioengineering9070271
F. L. Badillo, C. A. R. Hernández, B. M. Narváez, y Y. E. A. Trillos, “Redes neuronales convolucionales: un modelo de Deep Learning en imágenes diagnósticas. Revisión de tema”, Rev. Colomb. Radiol., vol. 32, no. 3, p. 5591, 2021. [En línea]. Disponible en: https://rcr.acronline.org/index.php/rcr/article/view/161
A. B. Schroeder, E. T. Dobson, C. T. Rueden, P. Tomancak, F. Jug, y K. W. Eliceiri, “The ImageJ ecosystem: Open-source software for image visualization, processing, and analysis”, Protein Sci., vol. 30, no. 1, pp. 234–249, 2021. [En línea]. Disponible en: https://doi.org/10.1002/pro.3993
L. Fernández, y P. Ramírez, “Procesamiento digital de imágenes para el análisis microbiológico: revisión y perspectivas”, Computación y Sistemas, vol. 25, no. 4, pp. 789–798, 2019. [En línea]. Disponible en: https://www.cys.cic.ipn.mx/ojs/index.php/CyS/article/view/3352
M. Roynard, E. Carlinet, y T. Géraud, “A Modern C++ Point of View of Programming in Image Processing”, Conf. Generative Programming: Concepts and Experiences, Auckland, Nueva Zelanda, pp. 164–171, 2022. [En línea]. Disponible en: https://doi.org/10.1145/3564719.3568692
M. Charbit, Ed., Digital Signal and Image Processing Using MATLAB, vol. 666. Hoboken, NJ, USA: John Wiley & Sons, 2010.
S. Gollapudi, Learn Computer Vision Using OpenCV: With Deep Learning CNNs and RNNs. Berkeley, CA: Apress, 2019.
M. Nixon y A. Aguado, Feature Extraction and Image Processing for Computer Vision, 5th ed. Academic Press, 2019.
M. Khan, S. Chakraborty, R. Astya, y S. Khepra, “Face detection and recognition using OpenCV”, Conf. Computing, Communication, and Intelligent Systems (ICCCIS), Greater Noida, India, pp. 116–119, 2019. [En línea]. Disponible en: https://doi.org/10.1109/ICCCIS48478.2019.8974493
H. Singh, Practical Machine Learning and Image Processing: For Facial Recognition, Object Detection, and Pattern Recognition Using Python, Berkeley, CA: Apress,2019.
A. A. Khan, A. A. Laghari, y S. A. Awan, “Machine Learning in Computer Vision: A Review”, EAI Endorsed Transactions on Scalable Information Systems, vol. 8, no. 32, Abr. 2021, [En línea]. Disponible en: https://doi.org/10.4108/eai.21-4-2021.169418
R. Szeliski, Computer Vision: Algorithms and Applications, 2nd ed. Cham, Switzerland: Springer Nature, 2022.
Published
How to Cite
Issue
Section
License
Copyright (c) 2026 Luis Alberto Jiménez Villa, María Salomé Alejandre Apolinar, Virginia Lagunes Barradas, Miguel Ángel Hidalgo Reyes

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
Licensing Agreement
This journal provides free access to its content through its website following the principle that making research available free of charge to the public supports a larger exchange of global knowledge.
Web content of the journal is distributed under a Attribution-NonCommercial-ShareAlike 4.0 International.
Authors may adopt other non-exclusive license agreements for the distribution of the version of the published work, provided that the initial publication in this journal is indicated. Authors are allowed and recommended to disseminate their work through the internet before and during the submission process, which can produce interesting exchanges and increase citations of the published work.

