Phylogenetic analysis of pathogenic isolates of the botryosphaeriaceae family in cacao (Theobroma cacao L.) in the Los Ríos area
DOI:
https://doi.org/10.18779/cyt.v15i2.583Keywords:
Cancro, cacao, ITS Region, EF1-a, philogeneAbstract
Phytosanitary problems caused by vascular fungi affecting CCN-51 cocoa plantations have become very important in recent decades, due to the damage they cause. Species of the Botryosphaeriaceae family are associated with cankers and drying of buds, downward death, fruit rot and neck rot, symptoms that have been observed in different areas and that reduce plant production. In order to characterize and identify pathogenic isolates of this fungus at the molecular level, five symptomatic trees were sampled from farms located in the cantons of: Buena Fe, Mocache, Quevedo, Quinsaloma and Valencia of the province of Los Rios, obtaining 15 isolates from which DNA was extracted and sequences from the ITS (Internal transcribed spacer) and EF1-a (Elongation factor of alpha) regions were used. Sequence sizes were verified by electrophoresis and putative identities of 3 species were established using the Basic local aligment tool (BLAST). Phylogenetic trees constructed using maximum likelihood and nearest neighbor algorithms, with isolates in five cantons, confirmed the presence of Lasiodiplodia theobromae in Quevedo, Diplodia seriata in Quinsaloma and Botryosphaeria dothidea in Valencia, Mocache and Buena Fe, highlighting a polytomy among the latter.
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Alvindia, D., & Gallema, F. (2017). Lasiodiplodia theobromae causes vascular streak dieback (VSD)–like symptoms of cacao in Davao Region, Philippines. Australasian Plant Disease Notes, 12, 54. https://doi.org/10.1007/s13314-017-0279-9
Barnett, H., & Hunter, B. (2010). Illustrated ggenera of imperfect fungi (1972 Burgess Publishing Company (ed.)).
Brooks, J. (2018). Probability concepts explained: Maximum likelihood estimation. Towards Data Science.
Caicedo, J. D., Lalangui, K. P., Pozo, A. N., Cevallos, P. A., Arahana, V. S., & Méndez, K. S. (2017). Multilocus molecular identification and phylogenetic analysis of Colletotrichum tamarilloi as the causal agent of Tamarillo (Solanum betaceum) anthracnose in the Ecuadorian highlands. European Journal of Plant Pathology, 148(4), 983–996. https://doi.org/10.1007/s10658-017-1155-3
Carbone, I., & Kohn, L. (2014). A method for designing primer sets for speciation studies in filamentous ascomycetes. Mycological Society of America, 91(3), 553–556.
Chou, C. (1988). Crown wilt of pinus radiata associated with diplodia pinea infection of woody stems. European Journal of Forest Pathology, 17(7), 398–411.
Crous, P., Slippers, B., Wingfield, M., Rheeder, J., Marasas, W., Philips, A., Alves, A., Burgess, T., Barber, P., & Groenewald, J. (2006). Phylogenetic lineages in the Botryospha … Phylogenetic lineages in the Botryosphaeriaceae Phylogenetic lineages in the Botryospha …. Mycology, 55, 235–253. http://dx.doi.org/10.1016/j.funbio.2016.11.001%0Ahttp://dx.doi.org/10.3114/ sim0020%0Ahttp://dx.doi.org/10.1016/j.funbio.2016.08.011%0Ahttp://www. mycosphere.org/pdf/Mycosphere_7_7_13.pdf%0Ahttp://www.
E. A, B., & F. F, H. (1981). Pathogenicity and histopathology of Botryosphaeria dothidea on apple stems. Phytopathology, 71(4), 375–379. https://doi.org/10.1094/phyto-71-375
Glass, N., & Donaldson, G. (1995). Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes. Applied and Environmental Microbiology, 61(4), 1323–1330. https://doi.org/10.1128/aem.61.4.1323-1330.1995
Guamán, M. (2018). Cararacterización morfológica, molecular y de resistencia a fungicidas de Colltotrichum sp., aislado de tomate de árbol (Solanum betaceum) [Universidad Central del Ecuador]. http://www.fao.org/3/I8739EN/i8739en.pdf%0Ahttp://dx.doi.org/10.1016/j. adolescence.2017.01.003%0Ahttp://dx.doi.org/10.1016/j.childyouth.2011.10.007 %0Ahttps://www.tandfonline.com/doi/full/10.1080/23288604.2016.1224023%0A http://pjx.sagepub.com/lookup/doi/10
Guerrero, G. (2015). El cacao ecuatoriano su historia empezó antes del siglo XV. Revista Líderes. https://www.revistalideres.ec/lideres/cacao-ecuatoriano-historia-empezo-siglo.html
KiWoo, K., EunWoo, P., & KyungKu, A. (1999). Pre-penetration behavior of Botryosphaeria dothidea on apple fruits. Plant Pathology Journal, 15(4), 223–227.
Marques, M., Lima, N., De Morais, M., Michereff, S., Phillips, A., & Câmara, M. (2013). Botryosphaeria, Neofusicoccum, Neoscytalidium and Pseudofusicoccum species associated with mango in Brazil. Fungal Diversity, 61, 195–208. https://doi.org/10.1007/s13225-013-0258-1
Mehl, J., Wingfield, M., Roux, J., & Slippers, B. (2017). Invasive everywhere? Phylogeographic analysis of the globally distributed tree pathogen Lasiodiplodia theobromae. Forests, 8(145), 1–22. https://doi.org/10.3390/f8050145
Palero, F., & Crandall, K. (2009). Phylogenetic inference using molecular data. 9–32. https://doi.org/10.1201/9781420092592-c5
Phillips, A. J. L., Alves, A., Abdollahzadeh, J., Slippers, B., Wingfield, M. J., Groenewald, J. Z., & Crous, P. W. (2013). The Botryosphaeriaceae: genera and species known from culture. Studies in Mycology, 76, 51–167. https://doi.org/10.3114/sim0021
Picos, P., García, R., León, J., Sañudo, A., & Allende, R. (2015). Lasiodiplodia theobromae en cultivos agrícolas de México: Taxonomía, Hospedantes, Diversidad y Control. Revista Mexicana de Fitopatología, 33(1), 54–74.
Pino, S. (2010). Ecuador the land of fine cocoa ‘Arriba’ (Vol. 7, Issue 1). https://www.researchgate.net/publication/269107473_What_is_governance/ link/548173090cf22525dcb61443/download%0Ahttp://www.econ.upf.edu/~ reynal/Civil wars_12December2010.pdf%0Ahttps://think-asia.org/handle/11540/8282%0Ahttps://www.jstor.org/stable/41857625
Rodríguez, E. (2013). Construcción de árboles filogenéticos (pp. 1–97). https://www.tamps.cinvestav.mx/~ertello/bioinfo/sesion13.pdf
Saitou, N., & Nei, M. (1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Molecular Biology and Evolution, 4(4), 406–425. https://doi.org/10.1093/oxfordjournals.molbev.a040454
Sandoval-Sánchez, M., Nieto-Ángel, D., Sandoval-Islas, J. S., Téliz-Ortiz, D., Orozco-Santos, M., & Silva-Rojas, H. V. (2013). Hongos asociados a pudrición del pedúnculo y muerte descendente del mango (Mangifera Indica L.). Agrociencia, 47, 61–73.
Santiago, D., Valadez, E., & Cibrián, D. (2015). Identificación molecular del complejo Botryosphaeria sp. asociado a cancros y secamiento de yemas en Eucalyptus sp. Revista Mexicana de Ciencias Forestales, 6(32), 93–106.
Schoch, C. L., Seifert, K. A., Huhndorf, S., Robert, V., Spouge, J. L., Levesque, C. A., Chen, W., & Fungal Barcoding Consortium. (2012). Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for fungi. PNAS, 109(16), 6241–6246. https://doi.org/10.1073/pnas.1117018109
Silva, P. (2018). Caracterización genotipica y de virulencia de Colletotrichum tamarilloi y Colletotrichum sp. causantes de la antracnosis en tomate de árbol. Universidad Central del Ecuador.
Slippers, B., & Wingfield, M. J. (2007). Botryosphaeriaceae as endophytes and latent pathogens of woody plants: diversity, ecology and impact. Fungal Biology Reviews, 21, 90–106. https://doi.org/10.1016/j.fbr.2007.06.002
Suarez, G. (2016). Botryosphaeriaceae asociadas a la muerte de ramas en plantaciones de Eucalyptus globulus Labill . en la región del Biobío y de La Araucania (Chile) [Universidad de Concepción]. In Universidad de Concepción. http://repositorio.udec.cl/bitstream/11594/2000/3/Tesis_Botryshaeriaceae _Asociadas_a_la_muerte_de_ramas.Image.Marked.pdf
Walker, J. (1980). The coelomycetes. Fungi imperfecto with Pycnidia, Acervuli and Stromala. Australasian Plant Pathology, 9(1), 120–121.
White, T. J., Bruns, T., Lee, S., & Taylor, J. (1990). Amplification and direct sequencing of fungal ribosomal rna genes for phylogenetics. PCR Protocols, 315–322. https://doi.org/10.1016/b978-0-12-372180-8.50042-1
Wright, A. F., & Harmon, P. F. (2010). Identification of species in the Botryosphaeriaceae family causing stem blight on southern highbush blueberry in Florida. Plant Disease, 94(8), 966–971. https://doi.org/10.1094/PDIS-94-8-0966
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