Effect of Ruta graveolens (Rue) essential oil in high concentrations on Staphylococcus aureus subsp aureus strain ATCC® 25904
DOI:
https://doi.org/10.18779/cyt.v18i1.790Keywords:
antimicrobial sensitivity, minimum inhibitory concentration, minimal bactericidal concentration, hydrodistillationAbstract
The objective of the research was to evaluate the antimicrobial efficacy of the essential oil of ruda (Ruta graveolens) onStaphylococcus aureus subp aureus strain ATCC ®25904. the plant was collected from the Inapisí sector of Constantino Fernández, the oil was obtained with the hydrodistillation technician, 10 concentrations were evaluated (10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 100% ) in dilution with double distilled water, each dilution was handled a total of 5 ml and tween 80 was added as 0,1% as emulsifier, the results showed that the determined minimum inhibitory concentration of the essential oil was defined at 30%; with respect to the Bactericidal minimum concentration evidenced by the zero growth of colonies on the plate, which was established at 40% concentration; the antimicrobial sensitivity study statistically show significant differences between treatments with the 0,5% Tukey test, the best being at 90% concentration with 25,25 mm of inhibition halo formation, however the treatments starting at 30% and showed significant halos formation, concluding that Ruta graveolens oil has antimicrobial efficacy on the strain evaluate.
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Al-Shuneigat, J. M., Al-Tarawneh, I. N., Al-Qudah, M. A., Al-Sarayreh, S.A., Al-Saraireh, Y. M. y Alsharafa, K. Y. (2015). The chemical composition and the antibacterial properties of Ruta graveolens L. Essential Oil Grown in Northern Jordan. Jordan Journal of Biological Sciences. 8(2), 139–143. https://doi.org/10.12816/0027560
Amabye, T. G. y Shalkh, T.M. (2015). Phytochemical Screening and Evaluation of Antibacterial Activity of Ruta graveolens L. - A Medicinal Plant Grown around Mekelle, Tigray, Ethiopia. Natural Products Chemistry y Research. 03(06): 1-4. https://doi.org/10.4172/2329-6836.1000195
Azalework, H.G., Sahabjada, Jafri. A., Arshad, M.D. y Malik, T. (2017). Phytochemical Investigation, Gc-Ms Profile and Antimicrobial Activity of a Medicinal Plant Ruta Graveolens L. From Ethiopia. International Journal of Pharmacy and Pharmaceutical Sciences. 9(6): 29–34. https://doi.org/10.22159/ijpps.2017v9i6.16812
Cerutti, M. y Neumayer, F. (2004). Introducción a la obtención de aceite esencial de limón. Invenio. 7(12): 149–155. https://www.redalyc.org/articulo.oa?id=87701214
Chaftar, N., Girardot, M., Labanowski, J., Ghrairi, T., Hani, K., Frere, J. y Imbert, C. (2015). Comparative evaluation of the antimicrobial activity of 19 essential oils. En Donelli, G. (eds), Advances in Microbiology, Infectious Diseases and Public Health. Advances in Experimental Medicine and Biology (Vol. 901, pp. 1-15). Springer, Cham. https://doi.org/10.1007/5584_2015_5011
Cockerill, F., Wikler, M., Alder, J., Dudley, M., Eliopoulos, G., Ferraro, M., Hardy, D., Hecht, D., Hindler, J., Jetel, D., Powell, M., Swenson, J., Thomson, R., Traczewski, M., Turnidge, J., Weinstein, M. y Zimmer, B. (2012). Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically; Approved Standard.
Conway, G. y Slocumb, J. (1979). Plants used as abortifacients and emmenagogues by Spanish New Mexicans. Journal of Ethnopharmacology. 1(3): 241–261. https://doi.org/10.1016/S0378-8741(79)80014-8
Delgadillo, L., Bañuelos, R., Delgadillo, O., Silva, M. y Gallegos, P. (2017). Composición química y efecto antibacteriano in vitro de extractos de Larrea tridentata, Origanum vulgare, Artemisa ludoviciana y Ruta graveolens. Nova Scientia, 9(19): 273. https://doi.org/10.21640/ns.v9i19.1019
Elansary, H., Szopa, A., Kubica, P., Ekiert, H., El-Ansary, D., Al-Mana, F. y Mahmoud, E. (2020). Polyphenol content and biological activities of Ruta graveolens L. and Artemisia abrotanum L. in northern Saudi Arabia. Processes. 8(5): 1–18. https://doi.org/10.3390/PR8050531
Ferhat, M., Kabouche, A. y Kabouche, Z. (2014). Comparative compositions of essential oils of three ruta species growing in different soils. Journal of Materials and Environmental Science. 5(3): 735–738. bit.ly/3TDgGyX
Franca, J. y Nascimento, A. (2015). Composition and antibacterial activity of Ruta graveolens L. (Rutaceae) volatile oils , from São Luís , Maranhão , Brazil. South African Journal of Botany. 99: 103–106. https://doi.org/10.1016/j.sajb.2015.03.198
Fredj, M., Marzouk, B., Chraief, I., Boukef, K. y Marzouk, Z. (2007). Analysis of Tunisian Ruta graveolens L. oils from Jemmel. Journal of Food, Agriculture and Environment. 5(1): 52–55. bit.ly/47xMMSw
Gallegos, M. (2016). Las plantas medicinales: principal alternativa para el cuidado de la salud, en la población rural de Babahoyo, Ecuador. Anales de La Facultad de Medicina. 77(4): 327. https://doi.org/10.15381/anales.v77i4.12647
García-Luján Concepción. (2006). Actividad antibacteriana de extractos vegetales en cepas hospitalarias de Staphylococcus aureus con resistencia múltiple. [Tesis de doctorado, Universidad Autónoma Agraria Antonio Narro]. bit.ly/3XQB0zz
Haddouchi, F., Chaouche, T., Zaouali, Y., Ksouri, R., Attou, A. y Benmansour, A. (2013). Chemical composition and antimicrobial activity of the essential oils from four Ruta species growing in Algeria. Food Chemistry. 141(1): 253–258. https://doi.org/10.1016/j.foodchem.2013.03.007
Hafezian, M., Asgarpanah, J. y Rahimifard, N. (2016). Chemical composition and antimicrobial activity of the essential oil from the endemic species pycnocycla bashagardiana Mozaff. Latin American Journal of Pharmacy. 35(7): 1634–1639. http://www.latamjpharm.org/resumenes/35/7/LAJOP_35_7_1_20.pdf
Jeyachandran, S., Oyyappan, D. y Baskaralingam, V. (2014). Discrete Nanoparticles of Ruta Graveolens Induces the Bacterial and Fungal Biofilm Inhibition. Cell Comunication & Adhesion. 21: 229–238. https://doi.org/10.3109/15419061.2014.926476
López-Rivera, R., Espinosa-Andrews, H., García-Márquez, E. y Herrera-Rodríguez, S. (2018). Efecto antifúngico de emulsiones a base de aceite esencial de orégano mexicano (Lippia graveolens), contra Candida albicans. Revista Salud Jalisco. 1: 42–45. http://www.medigraphic.com/pdfs/saljalisco/sj-2018/sj181g.pdf
Martínez, Y. y Gómez, L. (2013). Social impact of an intervention strategy for the rational prescription of natural medicines implemented in Céspedes during 2011. Revista Cubana de Plantas Medicinales. 18(4): 609–618. http://scielo.sld.cu/pdf/pla/v18n4/pla12413.pdf
Mejri. J., Abderrabba, M. y Mejri, M. (2010). Chemical composition of the essential oil of Ruta chalepensis L: Influence of drying, hydro-distillation duration and plant parts. Industrial Crops and Products. 32(3): 671–673. https://doi.org/10.1016/j.indcrop.2010.05.002
Ojala, T., Remes, S., Haansuu, P., Vuorela, H., Hiltunen, R., Haahtela, K. y Vuorela, P. (2000). Antimicrobial activity of some coumarin containing herbal plants growing in Finland. Journal of Ethnopharmacology. 73: 299–305. https://doi.org/10.1016/S0378-8741(00)00279-8
Pino-Pérez, O., Rojas-Fernández, M., Sánchez-Pérez, Y. y Espinosa-Castaño, I. (2018). Actividad antibacteriana de aceites esenciales obtenidos de plantas de origen cubano sobre Streptococcus suis. Revista de Salud Animal. 40(3): 1–5. http://scielo.sld.cu/pdf/rsa/v40n3/2224-4700-rsa-40-03-e03.pdf
Pino, O., Sánchez, Y., Roja, M., Abreu, Y., Correa, T., Martínez, D. y Montes de Oca, R. (2014). Composición Química y Actividad Antibacteriana del Aceite Esencial de Ruta Chalepensis L. Rev. Protección Veg. 29(3): 220–225. http://scielo.sld.cu/pdf/rpv/v29n3/rpv11314.pdf
Reddy, D. y Al-Rajab, A. (2016). Chemical composition , antibacterial and antifungal activities of Ruta graveolens L volatile oils. Cogent Chemistry. 2: 1–11. https://doi.org/10.1080/23312009.2016.1220055
Ríos, A., Barquero, M., Ortiz, G., Ayllón, T., Smit, L., Rodríguez-Dominguez, M. y Sánchez-Días, A. (2015). Staphylococcus multirresistentes a los antibióticos y su importancia en medicina veterinaria. Clin.Vet. Peq. Anima. 35(3): 149–161. https://www.clinvetpeqanim.com/img/pdf/223473834.pdf
Rodrigues, J., Melo, M. y Lima, E. (2008). In vitro antimicrobial activity of plants in Acute Otitis Externa. Rev.Bras Otorrinolaringol. 74(1): 118–124. https://doi.org/10.1016/S1808-8694(15)30761-8
Rojas, J., Mender, T., Rojas, L., Gullien, E., Buitrago, A., Lucena, M. y Cárdenas, N. (2011). Estudio comparativo de la composición química y actividad antibacteriana del aceite esencial de Ruta graveolens L. recolectada en los estados Mérida y Miranda, Venezuela. Avances En Química. 6(3): 89–93. https://www.redalyc.org/pdf/933/93321324005.pdf
Torrenegra-Alarcón, M., Granados-Conde, C., Durán-Lengua, M., León-Méndez, G., Yáñez-Rueda, X., Martínez, C. y Pájaro-Castro, N. (2016). Composición Química y Actividad Antibacteriana del Aceite Esencial de Minthostachys mollis. Orinoquia. 20(1): 69–74. https://www.redalyc.org/pdf/896/89647074008.pdf
Valles, M., Alvarado, P., Rodríguez, I., Saldaña, W., Reyes, W. y Vargas, A. (2014). Efecto del aceite esencial de Origanum vulgare en la supervivencia de Staphylococcus aureus, Salmonella thypi, Salmonella parathypi y Salmonella enteritidis en carne de cerdo pasteurizada y refrigerada. Revista Rebiol, 34(1): 57–68. https://revistas.unitru.edu.pe/index.php/facccbiol/article/view/589/551
Véliz-Jaime, M., González-Diaz, Y. y Martínez-Despaigne, Y. (2019). Technical and economic evaluation of the project to obtain essential oils. Tecnología Química. 39(1): 207–220. http://scielo.sld.cu/pdf/rtq/v39n1/2224-6185-rtq-39-01-207.pdf
Zekry, E., Mahmoud, R., El-Baky, A., Yehia, S., Abd, M., Hakeem, E., Mohamed, M. y Salah, M. (2018). Chemical composition and antimicrobial activities of essential oils of Ruta graveolens plants traeted with salicylic acid under drougt stress conditions. Future Journal of Pharmaceutical Sciences. 4(2): 254–264. https://doi.org/10.1016/j.fjps.2018.09.001
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Copyright (c) 2025 Mayra Andrea Montero Recalde, Carlos Enrique Aroca Izurieta, Álvaro Jeanpool Aroca Tirado, María Angélica Chuquimarca Chuquimarca, Julio César Pujos, Esteban Santiago Guevara
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