Prevalence and detection by nested PCR of Anaplasma marginale in cattle and tick in the center of the coast of Ecuador

Authors

  • Ariel Escobar Troya Universidad Técnica Estatal de Quevedo
  • Edgar Pinargote Mendoza Universidad Técnica Estatal de Quevedo
  • Helen Carranza Patiño Universidad Técnica Estatal de Quevedo
  • Mercedes Susana Carranza Patiño Universidad Técnica Estatal de Quevedo
  • Orly Fernando Cevallos Falquez Universidad Técnica Estatal de Quevedo
  • Patricio Villarreal Universidad de Guayaquil

DOI:

https://doi.org/10.18779/cyt.v8i1.145

Keywords:

DETECTION, CATTLE, FARMS, SALTING OUT.

Abstract

The bacterium causing anaplasmosis in cattle is known as Anaplasma marginale. It is mainly transmitted biologically by ticks, flies, and fomites; it can also be transmitted through infected blood as a result of improper use of surgical tools. Up to this moment, Ecuador lacks updated studies on efficient procedures for specific A. marginale diagnosis and eradication through the control of these insects, therefore it is necessary to develop and implement proposals related to the use of this molecular tool for potential vectors of transmission of this disease in cattle. For this work, DNA was extracted efficiently by the method of Salting Out. A total of 255 samples were analyzed by Nested PCR, distributed as follows |108| Rhipicephalus (Boophilus) microplus, |85| bovine, from these, the 13.46% and 85.48% were positive for rickettsia, the samples of Amblyomma spp |62| all were negative. The total Kappa index of infested cattle against Rhipicephalus (Boophilus) microplus|, was not significant (0.28). Subsequently,the presence or absence of disease which was determined by χ2 (p = 0.66) does not depend on the location from where the cattle comes

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Published

2015-09-15

How to Cite

Escobar Troya, A., Pinargote Mendoza, E., Carranza Patiño, H., Carranza Patiño, M. S., Cevallos Falquez, O. F., & Villarreal, P. (2015). Prevalence and detection by nested PCR of Anaplasma marginale in cattle and tick in the center of the coast of Ecuador. Ciencia Y Tecnología, 8(1), 11–17. https://doi.org/10.18779/cyt.v8i1.145

Issue

Vol. 8 No. 1 (2015): January-June (2015)

Section

Life Science

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