PROGRESS in vitro PROPAGATION Swietenia Macrophylla King (MAHOGANY)

Authors

  • Mercedes Susana Carranza Patiño Universidad Técnica Estatal de Quevedo
  • Ariel Escobar Troya Universidad Técnica Estatal de Quevedo
  • Héctor Reyes Morán Universidad Técnica Estatal de Quevedo
  • Jaime Morante Carriel Universidad Técnica Estatal de Quevedo
  • José Enrique Nieto Rodríguez Universidad Técnica Estatal de Quevedo
  • Maria Lorena Cadme Universidad Técnica Estatal de Quevedo
  • Orly Fernando Cevallos Falquez Universidad Técnica Estatal de Quevedo
  • Washington Fernando Mora Silva Universidad Técnica Estatal de Quevedo

DOI:

https://doi.org/10.18779/cyt.v6i2.133

Keywords:

PROTOCOL, IN VITRO PROPAGATION, SWIETENIA MACROPHYLLA, NODAL SEGMENTS.

Abstract

Mahogany (Swietenia macrophylla), is a widely used timber tree species valued for its hardness, strength, beauty and quality. Intensive explotation and inadequate use of this species have resulted in the loss of genetic variability, making impossible the implementation of breeding programs of mahogany in Ecuador. In vitro culture is a promising technique that would help reducing this problem by producing plants with high genetic strength. The objective of this research was to establish a method to facilitate in vitro propagation of mahogany from nodal segments. In order to prevent contamination, nodal segments were treated with Ca(ClO)2 solution for 20 min. Healthy explants were transferred to MS/2 culture medium with different concentrations of benzylaminopurine (BAP) and indolebutyric acid (IBA) for further in vitro proliferation, obtaining 70% of shoots on medium supplemented with 2 mg L-1 of BAP and 1 mg L-1 IBA. Bud treatment with different concentrations of ANA for in vitro rooting was not efficient, although the greatest survival rate (65%) was obtained on culture medium
containing 2 mg L-1 BAP and 1 mg L-1 NAA.

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Published

2014-05-12

How to Cite

Carranza Patiño, M. S., Escobar Troya, A., Reyes Morán, H., Morante Carriel, J., Nieto Rodríguez, J. E., Lorena Cadme, M., Cevallos Falquez, O. F., & Mora Silva, W. F. (2014). PROGRESS in vitro PROPAGATION Swietenia Macrophylla King (MAHOGANY). Ciencia Y Tecnología, 6(2), 1–8. https://doi.org/10.18779/cyt.v6i2.133

Issue

Vol. 6 No. 2 (2013): July-December (2013)

Section

Ciencias agrarias

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