SEROLOGICAL DIAGNOSIS (ROSE BENGAL) AND MOLECULAR (PCR) OF BRUCELLOSIS IN HUMAN

Authors

  • Orly Fernando Cevallos Falquez Universidad Técnica Estatal de Quevedo
  • Aldelmo Rodríguez Grefa Universidad Técnica Estatal de Quevedo
  • Ariel Escobar Troya Universidad Técnica Estatal de Quevedo
  • Camilo Mestanza Uquillas Universidad Técnica Estatal de Quevedo
  • Diego Romero Garaicoa Universidad Técnica Estatal de Quevedo
  • Fabricio Canchignia Martínez Universidad Técnica Estatal de Quevedo
  • Jaime Fabian Vera Chang Universidad Técnica Estatal de Quevedo
  • Jonathan Mariscal Álvarez Universidad Técnica Estatal de Quevedo
  • Ketty Cobeña Rosado Universidad Técnica Estatal de Quevedo
  • Luís Ramos Gavilanes Universidad Técnica Estatal de Quevedo
  • Maria Lorena Cadme Universidad Técnica Estatal de Quevedo
  • Mercedes Susana Carranza Patiño Universidad Técnica Estatal de Quevedo
  • Silvia Gicela Saucedo Aguiar Universidad Técnica Estatal de Quevedo
  • Ximena Reyes Chancay Universidad Técnica Estatal de Quevedo

DOI:

https://doi.org/10.18779/cyt.v3i1.86

Keywords:

BRUCELLA, SEROLOGY, PERIPHERAL BLOOD, ECUADOR.

Abstract

The objective of this research was: To determine the presence of brucellosis in the personnel working in the slaughterhouses of the cantons, Buena Fe, Quevedo, El Empalme and Pichincha, the serological test using Rose Bengal (RB) and molecular techniques ( PCR). Was used as a source of peripheral blood DNA and antibodies. Of a total of 115 blood samples collected at staff working in the slaughterhouses and slaughterers and operators, 54 (47%) and 15 (13%) were PCR positive and RB respectively, giving 61 (53%) and 100 (87%) negative for both tests accordingly. The average number of positive cases for the three populations except Pichincha by PCR was 3.4% and 39.4% RB. It takes care with the Pichincha Canton because 19 (70.3%) of samples were positive with RB of which 12 (44.4%) when analyzed with PCR were negative, and showed a 52.6% , indicating a high incidence of the disease. As long as the negative sample RB 8, 2 were PCR positive giving a correlation between negative 75%. The positive samples amplified a fragment of 725 pb. This work suggests that PCR is a highly efficient tool and very useful for diagnosis of brucellosis in humans.

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Published

2010-06-30

How to Cite

Cevallos Falquez, O. F., Rodríguez Grefa, A., Escobar Troya, A., Mestanza Uquillas, C., Romero Garaicoa, D., Canchignia Martínez, F., Vera Chang, J. F., Mariscal Álvarez, J., Cobeña Rosado, K., Ramos Gavilanes, L., Lorena Cadme, M., Carranza Patiño, M. S., Saucedo Aguiar, S. G., & Reyes Chancay, X. (2010). SEROLOGICAL DIAGNOSIS (ROSE BENGAL) AND MOLECULAR (PCR) OF BRUCELLOSIS IN HUMAN. Ciencia Y Tecnología, 3(1), 27–32. https://doi.org/10.18779/cyt.v3i1.86

Issue

Vol. 3 No. 1 (2010): January-June (2010)

Section

Ciencias agrarias

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