In vitro micro propagation of Nothofagus alpina through the use of phytohormones

Authors

  • Luz García Cruzatty Universidad Técnica Estatal de Quevedo
  • Betty González Osorio Universidad Técnica Estatal de Quevedo
  • Jaime Morante Carriel Universidad Técnica Estatal de Quevedo
  • Jessenia Castro Olaya Universidad Austral de Chile
  • Judith Carrasco Universidad Austral de Chile
  • Ricardo Luna Murillo Universidad Técnica Estatal de Quevedo

DOI:

https://doi.org/10.18779/cyt.v8i1.146

Keywords:

IN VITRO CULTURE, ORGANOGENESIS, CULTURE MEDIA, ROOTING, NOTHOFAGUS ALPINA.

Abstract

Nothofagus alpina is one of the most important forest species in the south of Chile and Argentina, where genetic improvement programs have been started. Micropropagation is a very  useful  technique when cloning  trees for improvement purposes, so  the objectives stated in this investigation were: to determine the best of 6-bencilaminopurine phytohormones (BAP) and the ideal culture medium to induce bud rooting and N. alpina nodal section sprouting. Murashige Skoog (MS) and Wood Plant (WPM) media were evaluated  with half macronutrients (rooting) and full micronutrients (multiplication), supplemented with 0.1% peptone, 2.0% saccharose, 0.7% agar and different auxin concentrations. MS medium supplemented with 2 mg L-1 AIB was found to be the best for inducing root formation in both number and  length of the main root. The greatest percentage of rooting was shown by WPM supplemented with 2 mg L-1 AIB. Treatments with cytokinin  for inducing multiplication originated calluses and failed to root, with a prevalence of calluses in WPM medium. The application of cytokinin is not indispensable for sprouting since in witness treatments, a greater number of buds was observed. This leads to the idea that the plant material used contains enough endogenous levels of this phytohormone.

Downloads

Download data is not yet available.

Downloads

Published

2015-09-15

How to Cite

García Cruzatty, L., González Osorio, B., Morante Carriel, J., Castro Olaya, J., Carrasco, J., & Luna Murillo, R. (2015). In vitro micro propagation of Nothofagus alpina through the use of phytohormones. Ciencia Y Tecnología, 8(1), 1–10. https://doi.org/10.18779/cyt.v8i1.146

Issue

Vol. 8 No. 1 (2015): January-June (2015)

Section

Life Science

License

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

Licensing Agreement

This journal provides free access to its content through its website following the principle that making research available free of charge to the public supports a larger exchange of global knowledge. 

Web content of the journal is distributed under a Attribution-NonCommercial-ShareAlike 4.0 International.

 

Authors may adopt other non-exclusive license agreements for the distribution of the version of the published work, provided that the initial publication in this journal is indicated. Authors are allowed and recommended to disseminate their work through the internet before and during the submission process, which can produce interesting exchanges and increase citations of the published work.